蛇骨骼肌纤维型及运动终板的研究：4—Di—2—ASP荧光染色及计算机图像分析     

何加Gan  高健 《科技通报》1997,13(4):253-257

用４－Ｄｉ－２－ＡＳＰ活性荧光染料染蛇腹横肌，清晰地显示三种肌纤维型及其运动终板，用ＨＰＳｃａｎＪｅｔ扫描仪对上述彩照进行高分辨扫描，得到良好的计算机数字图像，借助对相应参数的精确测定与分析，实现对形态学的直观定量表达  相似文献   

烟雾吸入伤早期氧化与抗氧化动态平衡变化的实验研究   总被引：1，自引：0，他引：1  

谢尔凡  杨宗城 《科技通报》1997,13(1):39-43

采用大鼠烟雾吸入伤模型，动态观察了伤后血浆、支气管肺泡灌洗液（ＢＡＬＦ）和肺组织中超氧化物歧化酶（ＳＯＤ）活性、丙二醛（ＭＤＡ）和共轭二烯（ＣＤ）含量的变化，辅以动脉血气分析、肺水量测定、ＢＡＬＦ中白细胞分类计数和病理检查．结果发现，伤后动物出现急性呼吸衰竭和严重肺水肿，肺内大量炎细胞聚集浸润；血浆、ＢＡＬＦ及肺组织中ＳＯＤ活性明显降低，ＣＤ和ＭＤＡ含量显著增加，ＭＤＡ／ＳＯＤ比值亦明显增高，且与ＢＡＬＦ中中性粒细胞数增加显著相关，提示氧化与抗氧化平衡失调可能在急性烟雾吸入性肺损伤的发生发展中起重要作用  相似文献   

Dietary restriction (DR) and its advantages     

M. N. Astagimath  Shrinivas B. Rao 《Indian journal of clinical biochemistry : IJCB》2004,19(1):1-5

Dietary restriction (DR) also called dietary control or calorie restriction is reported to have many advantages with regard to human health. It leads to suppression of obesity, mitigates free radicals and increases available antioxidants which are accounted for extending the life span of individuals. DR is also reported to induce synthesis of heat shock proteins in animals as a control mechanism against stress. Further, it is known to play a significant role in decreasing toxicity and lethality due to a variety of toxic chemicals and drugs by stimulating tissue repair damaged by the toxicants leading to restoration of intact organ and its functions. Moreover, extensive work done on animals indicate DR has an important role in suppressing certain types of cancer. In this review an effort is made to highlight the various advantages of DR from the point of human health perspective.  相似文献   

Effect of high glucose levels on the calcification of vascular smooth muscle cells by inducing osteoblastic differentiation and intracellular calcium deposition via BMP-2/Cbfα-1 pathway     

Liu F  Zhong H  Liang JY  Fu P  Luo ZJ  Zhou L  Gou R  Huang J 《Journal of Zhejiang University. Science. B》2010,11(12):905-911

In this paper,we investigate the effect and the possible mechanism of high glucose levels on the calcification of human aortic smooth muscle cells (HASMCs).HASMCs were divided into four groups: normal glucose group (NG),osmolality control group (OC),high glucose group (HG,HASMCs culture medium containing 30 mmol/L glucose),and high glucose plus recombinant human Noggin protein (bone morphogenetic protein-2 (BMP-2) antagonist) group (HN).The mRNA levels and the protein expressions of BMP-2 and core binding factor alpha-1 (Cbfα-1) were measured by real-time quantitative polymerase chain reaction (PCR) and Western blot.After induced by 10 mmol/L β-glycerol phosphoric acid,cells were harvested for assessments of alkaline phosphatase (ALP) activities at Days 1,2,and 3,and intracellular calcium contents at Days 7 and 14,respectively.High glucose levels increased the mRNA levels and the protein expressions of BMP-2 and Cbfα-1 (P0.05).The expression of Cbfα-1 was partially blocked by Noggin protein (P0.05),while BMP-2 was not (P0.05).After being induced by β-glycerol phosphoric acid,high glucose levels increased the ALP activity [(48.63±1.03) vs.(41.42±2.28) U/mg protein,Day 3;P0.05] and the intracellular calcium content [(2.76±0.09) vs.(1.75±0.07) μmol/mg protein,Day 14;P0.05] in a time-dependent manner when compared with the NG group,while the ALP activity could not be blocked by Noggin protein [(48.63±1.03) vs.(47.37±0.97) U/mg protein,Day 3;P0.05].These results show that high glucose levels can evoke the calcification of HASMCs by inducing osteoblastic trans-differentiation and intracellular calcium deposition via the BMP-2/Cbfα-1 pathway,which can be partially blocked by Noggin protein.  相似文献   

炙甘草汤对豚鼠心室肌细胞的电生理影响   总被引：1，自引：0，他引：1  

王雪芳  刘艳明  张晓云  张淑萍  张秀琴 《河北北方学院学报(医学版)》2007,24(1):20-22

目的：研究炙甘草汤对豚鼠心室肌细胞电生理影响。方法：应用常规的玻璃微电极细胞内记录技术，观察炙甘草汤对豚鼠心室肌细胞的动作电位0相幅值（APA）。动作电位时程（APD），50％复极化时间（APD50），90％复极化时间（APD90）的影响。结果：（1）用20mg／ml的炙甘草汤灌流后与正常对照组相比细胞电生理变化不明显；（2）用40mg／ml的炙甘草汤灌流后与正常对照组相比动作电位时程（APD）缩短（P〈0．05），90％复极化时间（APD90）下降（P〈0．05）；（3）用80mg／ml的炙甘草汤灌流后与正常对照组相比动作电位时程（APD）明显缩短（P〈0.01），50％复极化时间（APD50）、90％复极化时间（APD90）下降（P〈0．05）。结论：炙甘草汤能缩短豚鼠心室肌细胞动作电位时程及50％、90％复极化时间。  相似文献   

几种沉水植物光合作用产氧量的比较观察     

蒋梅兰  高枫 《台州学院学报》2001,(6)

在相同条件下,对几种不同沉水植物光合作用产氧量进行了比较观察。结果表明:金鱼藻、竹叶眼子菜是进行光合作用产氧量实验的好材料。  相似文献   

非蛋白呼吸商中糖和脂肪氧化百分比类型的确定     

冉辉 《铜仁学院学报》2002,4(4):77-80

非蛋白呼吸商是生理学中的一个重要概念，指的是糖的脂肪体内氧化时放出的CO2量与消耗的O2量的比值。对应不同的非蛋白呼吸商，糖和脂肪氧化的百分比不同。本通过定量计算分析表明，糖和脂肪氧化百分比既不是质量百分比，也不是放能百分比，而是耗氧百分比。  相似文献   

意大利蜜蜂(Apis mellifera L.)飞翔肌的发育     

叶巧真  卢爱平  张景强 《中山大学学报论丛》1995,(2)

全变态昆虫肌肉的转化与脊椎动物肌肉损伤修复有很大的相似之处。本文以意大利蜜蜂飞翔肌的发育阐明这一观点。两者的相似性表现为:①参与肌肉修复和成蜂飞翔肌形成的成肌细胞来源于胚细胞;②飞翔肌的发育过程中肌肉的转化和肌肉损伤修复均有幼虫残余肌丝或损伤部位残余肌丝和成肌细胞融合,成肌细胞与成肌细胞之间的融合等现象。这些结果为全变态昆虫肌肉的转化作为了解脊椎动物肌肉再生知识的便利模型的可行性,提供了理论依据。  相似文献   

浅析如何提高我国短跑运动员途中跑的能力     

薛红 《大连大学学报》2003,24(6):66-68,71

途中跑技术在田径短距离跑项目中是最重要的一个技术环节,对短跑成绩起决定作用. 目前,我国短跑运动停滞不前,关键一环是运动员途中跑的能力没有得到有效的提高.通过和世界优秀运动员的比较,从运动技术、运动能力、能量供应等不同角度,分析影响我国短跑运动员途中跑能力的问题所在,提出解决的有效途径,供大家在以后的教学训练中参考.  相似文献   

Programmed cell death features in apple suspension cells under low oxygen culture     

徐昌杰  陈昆松  FERGUSON Ian B 《Journal of Zhejiang University. Science. B》2004,(2)

INTRODUCTION A number of abiotic stresses have been shown to induce programmed cell death (PCD) responses in plant cells. High temperatures (e.g. 55 篊) induce nuclear condensation and DNA fragmentation in cultured carrot, cucumber and Arabidopsis cells (McCabe et al., 1997; McCabe and Leaver, 2000), and also mitochondrial cytochrome c release in cucumber cotyledons (Balk et al., 1999). The PCD pathway also appears to be activated by ozone. Ozone induces an oxidative burst with acc…  相似文献